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Title 

Stc1: a critical link between RNAi and chromatin modification required for heterochromatin integrity

Authors 

E H BayneS A WhiteA KaganskyD A BijosL Sanchez-PulidoKwang Lae HoeDong Uk KimH O ParkC P PontingJ RappsilberR C Allshire

Publisher 

Elsevier (Cell Press)

Issue Date 

2010

Citation 

Cell, vol. 140, no. 5, pp. 666-677

Keywords 

CELLBIOSIGNALNIG

Abstract 

In fission yeast, RNAi directs heterochromatin formation at centromeres, telomeres, and the mating type locus. Noncoding RNAs transcribed from repeat elements generate siRNAs that are incorporated into the Argonaute-containing RITS complex and direct it to nascent homologous transcripts. This leads to recruitment of the CLRC complex, including the histone methyltransferase Clr4, promoting H3K9 methylation and heterochromatin formation. A key question is what mediates the recruitment of Clr4/CLRC to transcript-bound RITS. We have identified a LIM domain protein, Stc1, that is required for centromeric heterochromatin integrity. Our analyses show that Stc1 is specifically required to establish H3K9 methylation via RNAi, and interacts both with the RNAi effector Ago1, and with the chromatin-modifying CLRC complex. Moreover, tethering Stc1 to a euchromatic locus is sufficient to induce silencing and heterochromatin formation independently of RNAi. We conclude that Stc1 associates with RITS on centromeric transcripts and recruits CLRC, thereby coupling RNAi to chromatin modification.

ISSN 

0092-8674

Link 

http://dx.doi.org/10.1016/j.cell.2010.01.038

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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