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Title 

A dual gold nanoparticle conjugate-based lateral flow assay (LFA) method for the analysis of troponin I

Authors 

Dong Hwan ChoiS K LeeYoung Kyeng OhB W BaeS D LeeS KimYong Beom ShinMin-Gon Kim

Publisher 

Elsevier

Issue Date 

2010

Citation 

Biosensors & Bioelectronics, vol. 25, no. 8, pp. 1999-2002

Keywords 

Lateral flow assayTroponin ITwo gold nanoparticle conjugates

Abstract 

For signal amplification without an additional operation step in a gold nanoparticle (AuNP)-based lateral flow assay (LFA), a new and simple method utilizing two AuNP-antibody conjugates was developed. The 1st conjugate was the AuNP immobilized with an anti-troponin I antibody and blocked with bovine serum albumin (BSA), and the 2nd conjugate was the AuNP immobilized with an anti-BSA antibody and blocked with human serum albumin. The two conjugates were encapsulated in different pads, respectively. A scheme of the LFA system is described in the part A of first figure. The size of the two conjugates was very critical in the detection sensitivity of troponin I. When 10 nm for the 1st and 40 nm for the 2nd were used, the detection sensitivity increased about a 100-fold compared to the conventional LFA. We could detect as low as 0.01 ng/mL troponin I in 10 min using the dual AuNP conjugate-based LFA, which was successfully applied in the analysis of serum samples of patients with myocardial infarction.

ISSN 

0956-5663

Link 

http://dx.doi.org/10.1016/j.bios.2010.01.019

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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