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Title 

A novel kanamycin/G418 resistance marker for direct selection of transformants in Escherichia coli and different yeast species

 

대장균과 다양한 효모에서 형질전환체를 선별할 수 있는 신규 항생제 저항성 마커

Authors 

M AgaphonovN RomanovaEui Sung ChoiM Ter-Avanesyan

Publisher 

Wiley-Blackwell

Issue Date 

2010

Citation 

Yeast, vol. 27, no. 4, pp. 189-195

Keywords 

Antibiotic resistanceCloning vectorGene disruptionTransformationYeast

Abstract 

We have developed a set of cloning vectors possessing a modified Tn903 kanamycin resistance gene that enables the selection of both kanamycin-resistant transformants in Escherichia coli and G418-resistant transformants in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha and Pichia pastoris. Expression of this gene in yeast is controlled by the H. polymorpha glyceraldehyde-3-phosphate dehydrogenase promoter, while expression in E. coli is governed by an upstream E. coli lacZ promoter. Applicability of the vectors for gene disruption in H. polymorpha and S. cerevisiae was demonstrated by inactivation of the HpMAL1 and URA3 genes, respectively. One of the vectors possesses a H. polymorpha ARS allowing plasmid maintenance in an episomal state. The small size of the vectors (2-2.5 kb) makes them convenient for routine DNA cloning. In addition, we report a novel approach for construction of gene disruption cassettes.

ISSN 

0749-503X

Link 

http://dx.doi.org/10.1002/yea.1741

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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