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Title 

Enhanced biomolecular detection based on localized surface plasmon resonance (LSPR) using enzyme-precipitation reaction

Authors 

Seung-Woo LeeJun Hyoung AhnMin-Gon KimYong Beom ShinJ J LeeK P LimK B Kim

Publisher 

American Scientific Publishers

Issue Date 

2010

Citation 

Journal of Nanoscience and Nanotechnology, vol. 10, no. 5, pp. 3246-3249

Keywords 

BiosensingEnzyme-Catalyzed precipitationLocalized surface plasmonNanodotNanoimprint lithography (NIL)

Abstract 

An enzyme-catalyzed precipitation reaction was employed as a means to increase the change in the LSPR signal after intermolecular bindings between antigens and antibodies occurred on gold nanodot surfaces. The gold nanodot array with an diameter of 175 nm and a thickness of 20 nm was fabricated on a glass wafer using thermal nanoimprint lithography. The human interleukin (hIL) 5 antibody was immobilized on the gold nanodot, followed by binding of hIL 5 to the anti-hIL 5. Subsequently, a biotinylated anti-hIL 5 and a alkaline phosphatase conjugated with streptavidin were simultaneously introduced. A mixture of 5-bromo-4-chloro-3-indolyl phosphate p-toluidine (BCIP) and nitro blue tetrazolium (NBT) was then used for precipitation, which resulted from the biocatalytic reaction of the alkaline phosphatase on gold nanodot. The LSPR spectra were obtained after each binding process. Using this analysis, the enzyme-catalyzed precipitation reaction on gold nanodots was found to be effective in amplifying the change in the peak wavelength of LSPR after molecular bindings.

ISSN 

1533-4880

Link 

http://dx.doi.org/10.1166/jnn.2010.2266

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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