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Title 

Monitoring of C-reactive protein using ion sensitive field effect transistor biosensor

Authors 

H J ParkS K KimKyoung Sook ParkSo Yeon YiJ W ChungBong Hyun ChungMoonil Kim

Publisher 

American Scientific Publishers

Issue Date 

2010

Citation 

Sensor Letters, vol. 8, no. 2, pp. 233-237

Keywords 

Antigen-antibody interactionBiosensorC-reactive proteinImmuno-FETISFET

Abstract 

An ion sensitive field effect transistor (ISFET) as a transducer, more precisely an immunologically modified-FET (immuno-FET) device, was utilized for the detection of the C-reactive protein (CRP), a potent marker for inflammation or cardiac risk. The ISFET biosensor investigated in this study was fabricated via a standard CMOS process. Anti-CRP monoclonal antibody was covalently attached to the gate surface using the self-assembled monolayer (SAM) method. The response of anti- CRP antibody to CRP was evaluated via the measurement of the electrical features of the ISFET device. As a consequence, a considerable reduction in drain current was clearly noted upon CRP binding to anti-CRP antibody on the gate, which can be explained by the charge effect, in which negatively charged CRP is predominantly responsible for the current drop in the n-type ISFET. The FET measurement was also verified by surface plasmon resonance (SPR) analysis. Collectively, our data convincingly showed that the FET-type biosensor is potentially applicable to the detection of CRP via antigen-antibody binding events on the gate surface.

ISSN 

1546-198X

Link 

http://dx.doi.org/10.1166/sl.20I0.1248

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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