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Title 

Signal amplification by enzyme reaction in an immunosensor based on localized surface plasmon resonance (LSPR)

Authors 

Tae Han LeeSeung-Woo LeeJee Ae JungJun Hyoung AhnMin-Gon KimYong Beom Shin

Publisher 

MDPI AG

Issue Date 

2010

Citation 

Sensors, vol. 10, no. 3, pp. 2045-2053

Keywords 

Enzyme-catalyzed precipitationGold nano-islandImmunosensorLocalized surface plasmon resonance (LSPR)

Abstract 

An enzymatic reaction was employed as a means to enhance the sensitivity of an immunosensor based on localized surface plasmon resonance (LSPR). The reaction occurs after intermolecular binding between an antigen and an antibody on gold nano-island (NI) surfaces. For LSPR sensing, the gold NI surface was fabricated on glass substrates using vacuum evaporation and heat treatment. The interferon-γ(IFN-γ) capture antibody was immobilized on the gold NIs, followed by binding of IFN-γ to the antibody. Subsequently, a biotinylated antibody and a horseradish peroxidase (HRP) conjugated with avidin were simultaneously introduced. A solution of 4-chloro-1-naphthol (4-CN) was then used for precipitation; precipitation was the result of the enzymatic reaction catalyzed the HRP on gold NIs. The LSPR spectra were obtained after each binding process. Using this method, the enzyme-catalyzed precipitation reaction on the gold NI surface was found to effectively amplify the change in the signal of the LSPR immunosensor after intermolecular binding.

ISSN 

1424-8220

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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