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Title 

Four different ways of alternative transcripts generation mechanism in ADRA1A gene

 

ADRA1A 유전자의 4개의 alternative 전사체의 생성 기작

Authors 

Jae Won HuhYoung Hyun KimSang Rae LeeDae Soo KimSang Je ParkH KimJi Su KimBong seok SongH S KimKyu Tae Chang

Publisher 

Genetics Society of Japan

Issue Date 

2010

Citation 

Genes and Genetic Systems, vol. 85, no. 1, pp. 65-73

Keywords 

Adra1a geneAlternative splicingSubstitutionTes integration

Abstract 

The ADRA1A (Alpha-1-adrenergic receptor) gene is one of the members of G protein-coupled receptor superfamily. Alternative splicing of this gene was known to generate four transcript variants which code four isoforms with various C-terminal regions. In this study, we conducted expression analysis and evolu- tionary characterization of alternative transcripts of the ADRA1A gene. In total, 10 alternative transcripts were identified using experimental approaches and in silico analysis. Among them, 6 alternative transcripts (T1, T2, T3, T4, T4-1, and T8) were validated by RT-PCR approaches and sequencing procedures. From the alternative splicing analysis, it could be assumed that there were three different alternative transcripts generation mechanisms and unknown mechanism. First one is the integration event of three different TEs (AluSc, L1MC5, and MIR3) as seen on the last exons of T3, T4, T4-1, T5, T6, and T7 transcripts. The second mechanism is a differential promoter usage on T8. The third one is a substitution event of the 3' splicing site during the primate evolution on T3. The last one is an unknown mechanism which was identified in the T4-1 transcript. Therefore, alternative transcripts of human ADRA1A gene occurred by four different ways, such as integration of TEs, differential promoter usage, substitution of splicing sites, and unknown mechanism.

ISSN 

1341-7568

Link 

http://dx.doi.org/10.1266/ggs.85.65

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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