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Title 

High sensitive detection of C-reactive protein by total internal reflection fluorescence microscopy on rapidly making nanoarray protein chip

Authors 

M S IslamHee Gu LeeJ ChooJ M SongS H Kang

Publisher 

Elsevier

Issue Date 

2010

Citation 

Talanta, vol. 81, no. 4, pp. 1402-1408

Keywords 

C-reactive proteinNanoarray protein chipSingle-molecule detectionSurface patterning toolTotal internal reflection fluorescence microscopy (TIRFM)

Abstract 

This study investigated a method for the high sensitivity detection and quantification of the C-reactive protein (CRP) in human serum using total internal reflection fluorescence microscopy (TIRFM) on a rapidly made nanoarray protein chip. The nanoarray biotin-probe was patterned onto 3-mercaptopropyl trimethoxysilane-coated cover glass with a spot diameter of ∼400 nm within 1 min using a NanoeNabler™-based surface patterning tool. The unlabeled CRP molecules were detected in human sera using TIRFM, based on a sandwich fluorescence immunoassay. The linear regression for standard CRP in the range of 50 zM-1 fM was determined using the equation y = 0.437x + 84.991 (R = 0.9993). This proposed method was ∼2000 times faster than conventional atomic force microscopy based dip-pen nanolithography in terms of the chip manufacturing process. Additionally this method was 6 × 106 times more sensitive than enzyme-linked immunosorbent assay and exhibited a wide dynamic linear range (50 zM-1 fM).

ISSN 

0039-9140

Link 

http://dx.doi.org/10.1016/j.talanta.2010.02.042

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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