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Title 

Time variation of fluorescence lifetime in enhanced cyan fluorescence protein

Authors 

S LeeS Y KimKyoungsook ParkJ JeongBong Hyun ChungS W Kim

Publisher 

Elsevier

Issue Date 

2010

Citation 

Journal of Luminescence, vol. 130, no. 7, pp. 1300-1304

Keywords 

ECFPLifetimeTCSPC

Abstract 

The lifetime variations of enhanced cyan fluorescence protein (ECFP) in relatively short integration time bins were studied via time-correlated single photon counting (TCSPC) measurement. We observed that minimum photon counts are necessary for the lifetime estimation to achieve a certain range of variance. The conditions to decrease the variance of lifetime were investigated and the channel width of the measurement of TCSPC data was found to be another important factor for the variance of lifetime. Though the lifetime of ECFP is best fit by a double exponential, a mono exponential fit for the same integration time is more stable. The results may be useful in the analysis of photophysical dynamics for ensemble molecules in short measurement time windows.

ISSN 

0022-2313

Link 

http://dx.doi.org/10.1016/j.jlumin.2010.02.043

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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