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Title 

Identification of a novel garlic cellulase gene

Authors 

Ae-Ri KimRyong Nam KimD W KimSang-Haeng ChoiAram KangSeong-Hyeuk NamHong-Seog Park

Publisher 

Springer Verlag (Germany)

Issue Date 

2010

Citation 

Plant Molecular Biology Reporter, vol. 28, no. 3, pp. 388-393

Keywords 

CellulaseCloningGarlicProtein expression

Abstract 

Genes encoding cellulase enzymes have been investigated in various plants due to the importance of cellulase enzymes in industrial applications, especially in the conversion of biomass into biofuels. Although several cellulase genes have been cloned and characterized, little is known about cellulase genes from garlic or enzyme activities of their gene products. In this study, a cellulase gene from garlic was cloned and characterized in gene and protein levels for the first time. The DNA sequence of the garlic cellulase gene showed 81% identity with the sequence of the endo-beta-1,4-glucanase of Pisum sativum. The open reading frame of this gene is 1,506 bp, which corresponds to 501 deduced amino acids. We identified the novel ORF region, which was translated into a 55.2 kDa protein using the protein expression vector, pET28a, in Escherichia coli and we confirmed that this protein has cellulase activity in vitro. Our study demonstrates that garlic is very useful, not only for the culinary and pharmaceutical industries, but also as an excellent natural source of various kinds of important genes and enzymes.

ISSN 

0735-9640

Link 

http://dx.doi.org/10.1007/s11105-009-0159-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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