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Title 

Signature gene expression profile of triclosan-resistant Escherichia coli

Authors 

Byung Jo YuJung-Ae KimJae Gu Pan

Publisher 

Oxford University Press (OUP)

Issue Date 

2010

Citation 

Journal of Antimicrobial Chemotherapy, vol. 65, no. 6, pp. 1171-1177

Keywords 

DNA microarrayE. coliFabITriclosan MIC

Abstract 

Objectives: To gain further insight into the defence mechanisms against triclosan in a mutant derived from an Escherichia coli strain carrying the triclosan-resistant target enzyme, FabI(G93V). Methods: An E. coli imp4231 FabI(G93V) strain was constructed by replacing intact fabI with a linear DNA cassette, fabI(G93V)-CmR, that contains a single mutation, GGT to GTT, at codon 93 of fabI(G93V) and a chloramphenicol resistance gene (CmR) as a marker for the mutant allele by a Red-mediated recombination system. Using this E. coli imp4231 FabI(G93V) strain, nitrosoguanidine (NTG) mutagenesis was performed to generate E. coli IFNs [imp4231 FabI(G93V) treated with NTG] displaying higher MICs of triclosan than its parent strain. The genes overexpressed in E. coli IFN4 were identified by DNA microarray analysis. Results: An E. coli imp4231 FabI(G93V) strain displays ~400-fold increased MICs of triclosan (MIC~8 mg/L) compared with the parent strain (MIC~0.02 mg/L). Furthermore, E. coli IFN4 has the highest MIC of triclosan (MIC~80 mg/L). DNA microarray analysis of E. coli IFN4 shows that many genes involved in the biosynthesis of membrane proteins, including transporters, reductases/dehydrogenases and stress response regulators, were highly expressed in the mutant. Conclusions: These results strongly indicate that E. coli IFN cells might protect themselves from triclosan by activating various defence mechanisms, such as (i) changing efflux activities; (ii) capturing the triclosan; and (iii) increasing the expression of important regulators or metabolic enzymes.

ISSN 

0305-7453

Link 

http://dx.doi.org/10.1093/jac/dkq114

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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