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Title 

A highly sensitive enzyme-amplified immunosensor based on a nanoporous niobium oxide (Nb2O5) electrode

 

다공성 니오븀산화물 전극을 이용한 고감도 효소증폭 면역센서

Authors 

Chang-Soo LeeDohyoung KwonJ E YooB G LeeJ ChoiBong Hyun Chung

Publisher 

MDPI AG

Issue Date 

2010

Citation 

Sensors, vol. 10, no. 5, pp. 5160-5170

Keywords 

Electrochemical biosensorEnzymeImmunosensorNiobium oxide

Abstract 

We report on the development of an enzyme-amplified sandwich-type immunosensor based on a thin gold film sputtered on an anodic nanoporous niobium oxide (Au@Nb2O5) electrode. The electrocatalytic activity of enzymatically amplified electroactive species and a stable electrode consisting of Au@Nb2O5 were used to obtain a powerful signal amplification of the electrochemical immunobiosensor. The method using this electrochemical biosensor based on an Au@Nb2O5 electrode provides a much better performance than those based on conventional bulk gold or niobium oxide electrodes. Our novel approach does not require any time-consuming cleaning steps to yield reproducible electrochemical signals. In addition, the strong adhesion of gold films on the niobium oxide electrodes offers a very stable substrate during electrochemical biosensing. Cyclic voltammetry measurements indicate that non-specific binding of proteins to the modified Au@Nb2O5 surface is sufficiently low to be ignored in the case of our novel system. Finally, we demonstrated the ability of the biosensor based on an Au@Nb2O5 offering the enhanced performance with a high resolution and sensitivity. Therefore, it is expected that the biosensor based on an Au@Nb2O5 has great potential for highly efficient biological devices.

ISSN 

1424-8220

Link 

http://dx.doi.org/10.3390/s100505160

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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