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Title 

Microglial peroxiredoxin V acts as an inducible anti-inflammatory antioxidant through cooperation with redox signaling cascades

Authors 

H N SunSun-Uk KimS M HuangJ M KimY H ParkS H KimH Y YangK J ChungT H LeeH S ChoiJ S MinM K ParkS K KimSang Rae LeeKyu Tae ChangS H LeeDae Yeul YuD S Lee

Publisher 

Wiley-Blackwell

Issue Date 

2010

Citation 

Journal of Neurochemistry, vol. 114, no. 1, pp. 39-50

Keywords 

C-jun N-terminal kinaseLipopolysaccharideMicrogliaNitric oxidePeroxiredoxin VReactive oxygen species

Abstract 

Reactive oxygen species (ROS) actively participate in microglia-mediated pathogenesis as pro-inflammatory molecules. However, little is known about the involvement of specific antioxidants in maintaining the microglial oxidative balance. We demonstrate that microglial peroxiredoxin (Prx) 5 expression is up-regulated by lipopolysaccharide (LPS) through activation of the ROS-sensitive signaling pathway and is involved in attenuation of both microglial activation and nitric oxide (NO) generation. Unlike in stimulation of oxidative insults with paraquat and hydrogen peroxide, Prx V expression is highly sensitive to LPS-stimulation in microglia. Reduction of ROS level by treatment with either NADPH oxidase inhibitor or antioxidant ablates LPS-mediated Prx V up-regulation in BV-2 microglial cells and is closely associated with the activation of the c-jun N-terminal kinase (JNK) signaling pathway. This suggests the involvement of ROS/JNK signaling in LPS-mediated Prx V induction. Furthermore, NO induces Prx V up-regulation that is ablated by the addition of inducible nitric oxide synthase inhibitor or deleted mutation of inducible nitric oxide synthase in LPS-stimulated microglia. Therefore, these results suggest that Prx V is induced by cooperative action among the ROS, RNS, and JNK signaling cascades. Interestingly, knockdown of Prx V expression causes the acceleration of microglia activation, including augmented ROS generation and JNK-dependent NO production. In summary, we demonstrate that Prx V plays a key role in the microglial activation process through modulation of the balance between ROS/NO generation and the corresponding JNK cascade activation.

ISSN 

0022-3042

Link 

http://dx.doi.org/10.1111/j.1471-4159.2010.06691.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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