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Title 

Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe

Authors 

Dong Uk KimJ HaylesD S KimV WoodH O ParkMi Sun WonHyang Sook YooT DuhigMi-young NamG PalmerS HanL JefferyS T BaekH leeY S ShimM LeeL KimK S HeoE J NohA R LeeY J JangKyung Sook ChungS J ChoiJ Y ParkYoung Woo ParkHwan Mook KimSong Kyu ParkH J ParkE J KangH B KimH S KangH M ParkK KimK SongK B SongP NurseKwang Lae Hoe

Publisher 

Nature Publishing Group

Issue Date 

2010

Citation 

Nature Biotechnology, vol. 28, no. 6, pp. 617-623

Keywords 

Cell cycleDeletion mutantsEssential geneEukaryotic cellsFission yeastGene deletionOrthologsRibosomal proteinsRNA polymeraseSchizosaccharomyces pombe

Abstract 

We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeastthe only other eukaryote for which a comprehensive knockout library existsrevealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.

ISSN 

1087-0156

Link 

http://dx.doi.org/10.1038/nbt.1628

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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