상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Monitoring of possible horizontal gene transfer from transgenic potatoes to soil microorganisms in the potato fields and the emergence of variants in Phytophthora infestans

 

유전자재조합 감자의 토양 미생물로 수평 유전자 이동 및 역병균 변종 출현 가능성 조사

Authors 

Sung Eun KimJae Sun MoonJung-kyu KimRan-hee YooWon Sik ChoiEun Na LeeS H LeeSung Uk Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2010

Citation 

Journal of Microbiology and Biotechnology, vol. 20, no. 6, pp. 1027-1031

Keywords 

AFLPEmerging variantsHorizontal gene transferSoil microorganismsTransgenic NDPK2 potatoes

Abstract 

To examine the possibility of horizontal gene transfer between transgenic potatoes and microorganisms in potato fields, the gene flow from transgenic potatoes containing the nucleoside diphosphate kinase 2 (NDPK2) gene to microorganisms in soils was investigated. The soil samples collected from the potato fields from March to October 2007 were examined by PCR, Southern hybridization, and AFLP fingerprinting. The NDPK2 gene from soil genomic DNAs was not detected by both PCR and Southern hybridization, indicating that gene transfer did not occur in the potato fields. In addition, no discrepancy was found in pathogenicity and noticeable changes for the appearance of variants of Phytophthora infestans in each generation when serial inoculations and the analysis of genomic DNAs by AFLP were conducted. Thus, these data suggest that transgenic potatoes do not give significant impacts on the communities of soil microorganisms and the emergence of variants, although continued research efforts may be necessary to make a decisive conclusion.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1002.02028

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)