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Title 

Cloning, expression and characterization of xylose isomerase, XylA, from Caldanaerobacter subterraneus subsp. yonseiensis

Authors 

Byoung Chan KimS N YuK Y KimJung-Sook LeeY R PyunS C Ahn

Publisher 

Springer Verlag (Germany)

Issue Date 

2010

Citation 

Biotechnology Letters, vol. 32, no. 7, pp. 929-933

Keywords 

Caldanaerobacter subterraneusThermoanaerobacterThermophileXylAXylose isomerase

Abstract 

The xylA gene, coding for xylose isomerase, from the extreme thermophile, Caldanaerobacter subterraneus subsp. yonseiensis was cloned, sequenced, and expressed in Escherichia coli. The nucleotide sequence of the xylA gene encoded a polypeptide of 438 residues with a calculated molecular weight of 50,170 Da. The purified XylA showed high sequence homology (92% identity) with that of Thermoanaerobacter thermohydrosulfuricus. The recombinant enzyme expressed in Escherichia coli was purified by heat treatment and gel chromatography. The purified enzyme was thermostable with optimal activity at 95°C. The enzyme required divalent cations including Zn2+ for its maximal activity and thermostability.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-010-0255-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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