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Title 

Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis

 

재조합효모를 이용한 글리세롤로부터 에탄올 생산

Authors 

Won Kyung HongChul Ho KimSun Yeon HeoL H LuoBaek Rock OhJeong Woo Seo

Publisher 

Springer Verlag (Germany)

Issue Date 

2010

Citation 

Biotechnology Letters, vol. 32, no. 8, pp. 1077-1082

Keywords 

Aldehyde dehydrogenase IIEthanol productionGlycerolHansenula polymorphaPyruvate decarboxylase

Abstract 

To improve production of ethanol from glycerol, the methylotrophic yeast Hansenula polymorpha was engineered to express the pdc and adhB genes encoding pyruvate decarboxylase and aldehyde dehydrogenase II from Zymomonas mobilis, respectively, under the control of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. The ethanol yield was 3.3-fold higher (2.74 g l-1) in the engineered yeast compared with the parent strain (0.83 g l-1). Further engineering to stimulate glycerol utilization in the recombinant strain via expression of dhaD and dhaKLM genes from Klebsiella pneumoniae encoding glycerol dehydrogenase and dehydroxyacetone kinase, respectively, resulted in a 3.7-fold increase (3.1 g l-1) in ethanol yield.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-010-0259-z

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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