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Title 

S. pombe genome deletion project: An update

Authors 

M SpirekZ BenkoM CarneckaC RumpfL CipakM BatovaI MarovaM NamDong Uk KimH O ParkJ HaylesKwang Lae HoeP NurseJ Gregan

Publisher 

Landes Bioscience

Issue Date 

2010

Citation 

Cell Cycle, vol. 9, no. 12, pp. 2399-2402

Keywords 

Essential genesFission yeastGene deletionGenomicsVectors

Abstract 

The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. The collection of deletion mutants now covers 99% of the fission yeast open reading frames.

ISSN 

1538-4101

Link 

http://dx.doi.org/10.4161/cc.9.12.11914

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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