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Title 

Fast microchip electrophoresis using field strength gradients for single nucleotide polymorphism identification of cattle breeds

Authors 

D OhI C CheongHee Gu LeeS K EoS H Kang

Publisher 

The Korean Chemical Society

Issue Date 

2010

Citation 

Bulletin of the Korean Chemical Society, vol. 31, no. 7, pp. 1902-1906

Keywords 

Cattle breedFast separationField strength gradientsMicrochip gel electrophoresisSingle nucleotide polymorphism (SNP)

Abstract 

A microchip electrophoresis (ME) method was developed using a programmed field strength gradients (PFSG) for the single nucleotide polymorphism (SNP) based fast identification of cattle breeds. Four different Korean cattle (Hanwoo) and Holstein SNP markers amplified by allele-specific polymerase chain reaction were separated in a glass microchip filled with 0.5% poly(ethyleneoxide) (Mr = 8 000 000) by PFSG as follows: 750 V/cm for 0 - 14 s, 166.7 V/cm for 14 - 31 s, 83.3 V/cm for 31 - 46 s, and 750 V/cm for 46 - 100 s. The cattle breeds were clearly distinguished within 45 s. The ME-PFSG method was 7 times and 5 times faster than the constant electric field ME method and the capillary electrophoresis- PFSG method, respectively, with a high resolving power (Rs = 5.05 - 9.98). The proposed methodology could be a powerful tool for the fast and simultaneous determination of SNP markers for various cattle breeds with high accuracy.

ISSN 

0253-2964

Link 

http://dx.doi.org/10.5012/bkcs.2010.31.7.1902

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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