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Title 

Dissociation mechanisms and implication for the presence of multiple conformations for peptide ions with arginine at the C-terminus: time-resolved photodissociation study

Authors 

S H YoonJeong Hee MoonM S Kim

Publisher 

Wiley-Blackwell

Issue Date 

2010

Citation 

Journal of Mass Spectrometry, vol. 45, no. 7, pp. 806-814

Keywords 

Arginine at C-terminusConformationMatrix effectNoncompetitive dissociationPeptide ion dissociation mechanismPhotodissociationTandem TOFTime-resolved photodissociation

Abstract 

Time-resolved photodissociation (PD) patterns of singly protonated peptides with arginine at the C-terminus (C-arg peptide ions) have been used to classify the dissociation channels into two categories, i.e. high-energy channels generating v, w and x and low-energy ones generating b, y and z. x + 1 formed by Cα-CO cleavage seems to be the intermediate ion in high-energy channels just as a + 1 is for N-arg peptide ions. Difference in time-resolved pattern indicates that the two sets of channels, high- and low-energy ones, are not in direct competition. Noncompetitive dissociation is also indicated by the observation of anomalous effect of matrix used in matrix-assisted laser desorption ionization, a cooler matrix generating more high-energy product ions both in spontaneous dissociation and in PD. Results from detailed investigation suggest that the two sets of channels start from two (ormore) different conformations.

ISSN 

1076-5174

Link 

http://dx.doi.org/10.1002/jms.1773

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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