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Title 

Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis

 

고초균에서 자동유도 발현시스템 개발

Authors 

S J LeeJae Gu PanSeung Hwan ParkSoo Keun Choi

Publisher 

Elsevier

Issue Date 

2010

Citation 

Journal of Biotechnology, vol. 149, no. 1, pp. 16-20

Keywords 

AutoinductionBacillus SubtilisBacillus thuringiensisConsensus sequenceCry genesCrystal proteinsDry cellsExpression levelsExpression systemExtracellular enzymesHigh level expressionHigher yieldPromoter sequencesProtein expressionsStationary phaseSubtilis

Abstract 

Bacillus thuringiensis produces crystal proteins (Cry) that account for up to 25% of the dry cell weight during the stationary phase. The high-level expression and stationary phase-specific autoinduction of the cry gene led to development of a cry promoter-based Bacillus expression system. Among the various cry promoters, cry3Aa promoter was selected by comparing the lacZ expression levels in Bacillus subtilis. An extracellular enzyme cellulase was highly upregulated during the stationary phase while under control of the cry3Aa promoter. Improvement of the cry3Aa promoter was obtained by modification of the promoter sequence. Specifically, a 5-fold increase in lacZ expression was obtained by changing both the -35 and -10 boxes of the cry3Aa promoter to the consensus sequence of the σA-dependent promoter of B. subtilis. The modified cry3Aa promoter produced a significantly higher yield of AprE, which suggests that the promoter may be useful for high-level protein expression in B. subtilis.

ISSN 

0168-1656

Link 

http://dx.doi.org/10.1016/j.jbiotec.2010.06.021

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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