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Title 

Isolation and functional analysis of cDNAs similar to Hyp-1 involved in hypericin biosynthesis from Hypericum erectum

Authors 

M L JinJ C AhnB HwangHong-Seog ParkH S LeeD W Choi

Publisher 

Springer Verlag (Germany)

Issue Date 

2010

Citation 

Biologia Plantarum, vol. 54, no. 4, pp. 725-729

Keywords 

amino acid sequenceESTsHPLCRT-PCRTLC

Abstract 

Hypericin, a naphthodianthrone, has been identified as the principal active compound found in St. John's wort (Hypericum perforatum L.). To generate a gene resource for hypericin and other valuable metabolites, we generated expressed sequence tags (ESTs) from H. erectum. Analyses of the ESTs enabled us to select three cDNAs, HeHyp1, HeHyp2, and HeHyp3, evidencing significant sequence homology to Hyp-1 that were involved in hypericin biosynthesis from H. erectum. The deduced amino acid sequence of HeHyp1 cDNA exhibits 95 % identity with Hyp-1. The HeHyp2 and HeHyp3 polypeptides also exhibit 81.1 % identity with Hyp-1. The transcripts of HeHyp1, HeHyp2, and HeHyp3 were detected in the root, stem, leaf, flower, and callus cells. Study using recombinant protein suggests that Hyp-1, HeHyp2, and HeHyp3 may be involved in the biosynthetic of hypericin or other emodin derivatives.

ISSN 

0006-3134

Link 

http://dx.doi.org/10.1007/s10535-010-0129-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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