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Title 

Expression and immunogenicity of a recombinant chimeric protein of human colorectal cancer antigen GA733-2 and an Fc antibody fragment in stably transformed Drosophila melanogaster S2 cells

Authors 

Y J SeokK I KimK H YooJ Hwang-BoH H LeeD H ShonK S KoH S KangK J LeeDoo-Byoung OhY H JoungI S Chung

Publisher 

Humana Press

Issue Date 

2010

Citation 

Applied Biochemistry and Biotechnology, vol. 162, no. 5, pp. 1435-1445

Keywords 

Cancer antigenDrosophila melanogaster S2 cellsExpressionImmunogenicityRecombinant GA733-2-Fc

Abstract 

Human colorectal cancer antigen GA733-2 fused to the immunoglobin Fc fragment (GA733-2-Fc) was expressed in stably transformed Drosophila melanogaster S2 cells, and the immunogenicity of recombinant GA733-2-Fc was investigated in mice. Recombinant GA733-2-Fc was secreted into a culture medium with a molecular mass of approximately 65 kDa. Recombinant GA733-2-Fc was purified to homogeneity using affinity fractionation with Protein A sepharose 4 Fast Flow. Recombinant GA733-2-Fc proteins elicited production of specific antibodies against recombinant GA733-2 by immunization through an intraperitoneal route. Recombinant GA733-2-Fc-induced antibodies showed a binding activity to human colorectal carcinoma HCT-116 cells. Secretory recombinant GA733-2-Fc from Drosophila S2 cell systems can be used as an effective experimental antigen for research in cancer vaccine development.

ISSN 

0273-2289

Link 

http://dx.doi.org/10.1007/s12010-010-8909-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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