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Title 

Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities

Authors 

H J RyuX JinJ H LeeH J WooYoung Min KimG J KimE S SeoH K KangJ KimD L ChoA KimuraD Kim

Publisher 

Elsevier

Issue Date 

2010

Citation 

Enzyme and Microbial Technology, vol. 47, no. 5, pp. 212-215

Keywords 

DextranaseDextransucraseFusion enzymeIsomalto-oligosaccharideResponse surface methodology

Abstract 

Long isomalto-oligosaccharides (IMOs) were generated via an engineered fusion enzyme of dextransucrase and dextranase (DSXR). To increase the expression level, response surface methodology (RSM) was utilized for optimization of protein expression conditions for enhancement of protein production by the effects of three-level-three-factors and their mutual interaction in Escherichia coli. Seventeen experiments were designed and conducted for investigation of cell density to start induction, induction temperature, and induction time. Optimal induction conditions included a cell density to start induction (A600) of 0.76 at 12.16°C for 18h for dextransucrase activity and a cell density to start induction (A600) of 0.75 at 10.5°C for 20.9h for dextranase activity. The produced dextransucrase or dextranase activity was obtained at 120.1±7.2 or 871±58U, respectively, from 1L cultures.

ISSN 

0141-0229

Link 

http://dx.doi.org/10.1016/j.enzmictec.2010.07.006

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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