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Title 

Evaluating the persistence of DNA from decomposing transgenic watermelon tissues in the field

 

형질전환 수박 DNA의 지속성 평가

Authors 

B LeeJi-Young ParkKee Woong ParkC H HarnHwan Mook KimChang-Gi Kim

Publisher 

The Botanical Society of Korea

Issue Date 

2010

Citation 

Journal of Plant Biology, vol. 53, no. 5, pp. 338-343

Keywords 

Citrullus lanatusDNA persistenceSoilTransgenic cropWatermelon

Abstract 

To analyze the persistence of the 35S promoter, nos terminator, and hpt, we buried the leaves and rootstocks of transgenic watermelons (Citrullus lanatus (Thunb.) Matsum. & Nakai) in 10 cm of soil. Qualitative and quantitative PCR analyses showed that the amount of transgenes in leaf samples was greatly decreased, by 70%, after 1 month, and only 2.5% remained after 2 months. No transgenes were detected in the leaves after 3 months. For buried rootstock samples, transgenes also degraded quickly, but a very small amount was still detectable up to 3 months later. In our investigation of possible gene transfer from decomposing transgenic watermelon to soil bacteria, only the 35S promoter was detected. However, further examination using colony dot hybridization tests indicated that such a transfer did not occur.

ISSN 

1226-9239

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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