상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Molecular switching fluorescence based high sensitive detection of label-free C-reactive protein on biochip

Authors 

S IslamH YuHee Gu LeeS H Kang

Publisher 

Elsevier

Issue Date 

2010

Citation 

Biosensors and Bioelectronics, vol. 26, no. 3, pp. 1028-1035

Keywords 

BiochipC-reactive protein (CRP)Label-free detectionMolecular switching fluorescenceTotal internal reflection fluorescence microscopy (TIRFM)

Abstract 

A novel detection technique on biochip for the quantification of label-free C-reactive protein (CRP) based on molecular switching of fluorescence (MSF) is demonstrated by total internal reflection fluorescence microscopy. It alters fluorescence intensity of fluoreseinamine isomer 1 (FAI) upon binding with its specific ligand, O-phosphorylethanolamine (PEA). In the MSF-based detection, FAI was used as an ink, printed on a 3-glycidoxypropyl-trimethoxysilane (GPTS)-coated glass coverslip. With the addition of GPTS conjugated PEA solution to the FAI-printed coverslip, the fluorescence intensity was remarkably decreased. Addition of CRP increased fluorescence intensity linearly in the range of 800. aM to 500. fM (R=0.997). The MSF-based biochip assay for the estimation of CRP in human sera showed ~200 times increased detection sensitivity in less than a third of the time to obtain results using a conventional enzyme-linked immunosorbent assay. This biochip detection is a promising new technique for the quantification of CRP molecules from trace amounts of clinical samples

ISSN 

0956-5663

Link 

http://dx.doi.org/10.1016/j.bios.2010.08.045

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)