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Title 

Antioxidant effects of the chestnut (Castanea crenata) inner shell extract in t-BHP-treated HepG2 cells, and CCl4- and high-fat diet-treated mice

Authors 

Jung-Ran NohGil-Tae GangYong Hoon KimK J YangJung Hwan HwangHyun Sun LeeW K OhK S SongChul Ho Lee

Publisher 

Elsevier

Issue Date 

2010

Citation 

Food and Chemical Toxicology, vol. 48, no. 11, pp. 3177-3183

Keywords 

Antioxidant effectCCl4Chestnut inner shell extractHigh-fat dietT-BHP

Abstract 

The antioxidant effects of chestnut inner shell extract (CISE) were investigated in a tert-butylhydroperoxide (t-BHP)-treated HepG2 cells, and in mice that were administered carbon tetrachloride (CCl4) and fed a high-fat diet (HFD). Pre-incubation with CISE significantly blocked the oxidative stress induced by t-BHP treatment in HepG2 cells (P<0.05) and preserved the activities of catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase compared to group treated with t-BHP only. Similarly, the CCl4- and HFD-induced reduction of antioxidant enzymes activities in liver was prevented by CISE treatment compared to control groups. Furthermore, hepatic lipid peroxidation were remarkably lower (P<0.05) in the CISE-treated groups with t-BHP or HFD. To determine the active compound of CISE, the fractionation of CISE has been conducted and scoparone and scopoletin were identified as main compounds. These compounds were also shown to inhibit the t-BHP-induced ROS generation and reduction in antioxidant enzyme activity in an in vitro model system. From these results, it was demonstrated that CISE has the ability to protect against damage from oxidative stressors such as t-BHP, CCl4 and HFD in in vitro and in vivo models. The CISE might be useful for the prevention of oxidative damage in liver cells and tissues.

ISSN 

0278-6915

Link 

http://dx.doi.org/10.1016/j.fct.2010.08.018

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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