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Title 

Eupafolin, a flavonoid isolated from Artemisia princeps, induced apoptosis in human cervical adenocarcinoma HeLa cells

Authors 

K S ChungJ H ChoiN I BackM S ChoiE K KangH G ChungTae Sook JeongK T Lee

Publisher 

Wiley-Blackwell

Issue Date 

2010

Citation 

Molecular Nutrition & Food Research, vol. 54, no. 9, pp. 1318-1328

Keywords 

ApoptosisBcl-2CaspaseEupafolinMitochondria

Abstract 

Although eupafolin, a flavone found in Artemisia princeps Pampanini, has been shown to inhibit the growth of several human cancer cells, its mode of action is poorly understood. In this study, we investigated the pro-apoptotic activities of eupafolin in human cervical carcinoma HeLa cells. It was found that eupafolin induced apoptosis in a dose-dependent manner, as evidenced by DNA fragmentation and the accumulation of positive cells for annexin V. In addition, eupafolin triggered the activations of caspases-3, -6, -7, -8, and -9 and the cleavages of their substrates, such as, poly (ADP-ribose) polymerase and lamin A/C. Furthermore, treatment with eupafolin resulted in a loss of mitochondrial membrane potential (ΔΨm), increased the release of cytochrome c to the cytosol, and altered the expression levels of B-cell lymphoma 2 (Bcl-2) family proteins. Interestingly, caspase-8, an initiator caspase, was activated after the loss of ΔΨm and the activations of caspases-3 and -9. Moreover, treatment with z-DEVD-fmk (a specific caspase-3 inhibitor) and the overexpression of Bcl-2 prevented eupafolin-stimulated caspase-8 activation. Altogether, these results suggest that the eupafolininduced apoptosis in HeLa cells is mediated by caspase-dependent pathways, involving caspases-3, -9, and -8, which are initiated by the Bcl-2-dependent loss of ΔΨm.

ISSN 

1613-4125

Link 

http://dx.doi.org/10.1002/mnfr.200900305

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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