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Title 

Random breakup of microdroplets for single-cell encapsulation

Authors 

E UmSeung Goo LeeJ K Park

Publisher 

American Institute of Physics

Issue Date 

2010

Citation 

Applied Physics Letters, vol. 97, no. 15, pp. 153703-153703

Abstract 

Microfluidic droplet-based technology enables encapsulation of cells in the isolated aqueous chambers surrounded by immiscible fluid but single-cell encapsulation efficiency is usually less than 30%. In this letter, we introduce a simple microgroove structure to break droplets into random sizes which further allows collecting of single-cell [Escherichia coli (E. coli)] containing droplets by their size differences. Pinched-flow separation method is integrated to sort out droplets of certain sizes which have high probability of containing one cell. Consequently, we were able to obtain more than 50% of droplets having single E. coli inside, keeping the proportion of multiple-cell containing droplets less than 16%.

ISSN 

0003-6951

Link 

http://dx.doi.org/10.1063/1.3500980

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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