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Title 

Alu-derived old world monkeys exonization event and experimental validation of the LEPR gene

Authors 

Jae Won HuhYoung Hyun KimDae Soo KimSang Je ParkSang Rae LeeSang-Hyun KimEkyune KimSun-Uk KimM S KimH S KimKyu Tae Chang

Publisher 

Springer Verlag (Germany)

Issue Date 

2010

Citation 

Molecules and Cells, vol. 30, no. 3, pp. 201-207

Keywords 

alternative transcriptAluJbexonization eventleptin receptortransposable elements

Abstract 

The leptin receptor (LEPR) is a crucial regulatory protein that interacts with Leptin. In our analysis of LEPR, novel AluJb-derived alternative transcripts were identified in the genome of the rhesus monkey. In order to investigate the occurrence of AluJb-derived alternative transcripts and the mechanism underlying exonization events, we conducted analyses using a number of primate genomic DNAs and adipose RNAs of tissue and primary cells derived from the crab-eating monkey. Our results demonstrate that the AluJb element has been integrated into our common ancestor genome prior to the divergence of simians and prosimians. The lineage-specific exonization event of the LEPR gene in chimpanzees, orangutans, and Old World monkeys appear to have been accomplished via transition mutations of the 5′ splicing site (second position of C to T). However, in New World monkeys and prosimians, the AluJb-related LEPR transcript should be silenced by the additional transversion mutation (fourth position of T to G). The AluJb-related transcript of human LEPR should also be silenced by a mutation of the 5′ splicing site (first position of G to A) and the insertion of one nucleotide sequence (minus fourth position of A). Our data suggests that lineage-specific exonization events should be determined by the combination event of the formation of splicing sites and protection against site-specific mutation pressures. These evolutionary mechanisms could be major sources for primate diversification.

ISSN 

1016-8478

Link 

http://dx.doi.org/10.1007/s10059-010-0108-x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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