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Title 

Proteomic analysis of oxidative stress-induced neuronal cell death by using two-dimensional fluorescence difference gel electrophoresi

Authors 

Eun Young KimTae-Sung YoonYoung Jae BahnDae Gwin JeongM R ParkSang Jeon ChungSung Goo ParkByoung Chul ParkSang Chul LeeS E RyuKwang-Hee Bae

Publisher 

Spandidos Publications

Issue Date 

2010

Citation 

International Journal of Molecular Medicine, vol. 26, no. 6, pp. 829-835

Abstract 

Oxidative stress has been implicated in a number of neurological disorders, including cerebral ischemia and neuro-degenerative diseases. Comprehensive proteomic studies were carried out using an immortalized mouse hippocampal cell line, HT22, exhibiting oxidative stress-mediated cell death upon glutamate treatment. Two-dimensional fluorescence difference gel electrophoresis (2D DIGE) of subcellular organelle fractions revealed that significant numbers of proteins showed quantitative changes during HT22 cell death, among which a total of 51 proteins were identified by mass spectrometry. The identified proteins indicate that HT22 cell death occurs through perturbations in mitochondrial function, changes in translational elongation machinery, and translocation of proteins across subcellular organelles. This list of proteins may shed light on oxidative stress-mediated neuronal cell death.

ISSN 

1107-3756

Link 

http://dx.doi.org/10.3892/ijmm-00000531

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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