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Title 

Development of a nanoparticle-based FRET sensor for ultrasensitive detection of phytoestrogen compounds

Authors 

A B DumbrepatilSeung Goo LeeSang Jeon ChungMyung Kyu LeeByoung Chul ParkT J KimEui-jeon Woo

Publisher 

Royal Society of Chemistry

Issue Date 

2010

Citation 

Analyst, vol. 135, no. 11, pp. 2879-2886

Abstract 

Phytoestrogens are plant compounds that mimic the actions of endogenous estrogens. The abundance of these chemicals in nature and their potential effects on health require the development of a convenient method to detect phytoestrogens. We have developed a nanoparticle (NP)-conjugated FRET probe based on the human estrogen receptor α (ER) ligand-binding domain (LBD) to detect phytoestrogens. The NP-conjugated FRET probe showed fluorescence signals for genistein, resveratrol and daidzein compounds with Δ ratios of 1.65, 2.60 and 1.37 respectively, which are approximately six times greater compared to individual FRET probes. A significantly higher signal for resveratrol versus genistein and daidzein indicates that the probe can differentiate between antagonistic phytoalexin substances and agonistic isoflavone compounds. NP-conjugated probes demonstrated a wide dynamic range, ranging from 10 -18 to 10-1 M with EC50 values of 9.6 × 10-10, 9.0 × 10-10 and 9.2 × 10-10 M for genistein, daidzein and resveratrol respectively, whereas individual probes detected concentrations of 10-13 to 10-4 M for phytoestrogens compounds. The time profile revealed that the NP-conjugated probe is stable over 30 h and there is not a significant deviation in the FRET signal at room temperature. These data demonstrate that conjugation of a FRET probe to nanoparticles is able to serve as an effective FRET sensor for monitoring bioactive compounds with significantly increased sensitivity, dynamic range and stability.

ISSN 

0003-2654

Link 

http://dx.doi.org/10.1039/c0an00385a

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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