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Title 

The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis

Authors 

H S KimS J KimN AbbasiR A BressanD J YunS D YooSuk Yoon KwonS B Choi

Publisher 

Wiley-Blackwell

Issue Date 

2010

Citation 

Plant Journal, vol. 64, no. 3, pp. 524-535

Keywords 

Activation tagging mutantDof transcription factorDof5.1Leaf polarityRevoluta

Abstract 

Dof proteins are transcription factors that have a conserved single zinc finger DNA-binding domain. In this study, we isolated an activation tagging mutant Dof5.1-D exhibiting an upward-curling leaf phenotype due to enhanced expression of the REV gene that is required for establishing adaxialabaxial polarity. Dof5.1-D plants also had reduced transcript levels for IAA6 and IAA19 genes, indicating an altered auxin biosynthesis in Dof5.1-D. An electrophoretic mobility shift assay using the Dof5.1 DNA-binding motif and the REV promoter region indicated that the DNA-binding domain of Dof5.1 binds to a TAAAGT motif located in the 5′-distal promoter region of the REV promoter. Further, transient and chromatin immunoprecipitation assays verified binding activity of the Dof5.1 DNA-binding motif with the REV promoter. Consistent with binding assays, constitutive over-expression of the Dof5.1 DNA-binding domain in wild-type plants caused a downward-curling phenotype, whereas crossing Dof5.1-D to a rev mutant reverted the upward-curling phenotype of the Dof5.1-D mutant leaf to the wild-type. These results suggest that the Dof5.1 protein directly binds to the REV promoter and thereby regulates adaxialabaxial polarity.

ISSN 

0960-7412

Link 

http://dx.doi.org/10.1111/j.1365-313X.2010.04346.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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