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Title 

Expression profiling of calcium induced genes in cultured human keratinocytes

Authors 

J S LeeMi-rang KimNam-Soon KimYong Sung KimJ M YangA Y ChoY LeeC D KimJ H Lee

Publisher 

Korean Academy of Medical Sciences

Issue Date 

2010

Citation 

Journal of Korean Medical Science, vol. 25, no. 4, pp. 619-625

Keywords 

CalciumGene expression profilingKeratinocyte differentiationNormalized library

Abstract 

Terminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. To examine the gene expression profile in calcium-induced keratinocyte differentiation, we constructed a normalized cDNA library using mRNA isolated from these calcium-treated keratinocytes. After sequencing about 10,000 clones, we were able to obtain 4,104 independent genes. They consisted of 3,699 annotated genes and 405 expressed sequence tags (ESTs). Some were the genes involved in constituting epidermal structures and others were unknown genes that are probably associated with keratinocytes. In particular, we were able to identify genes located at the chromosome 1q21, the locus for the epidermal differentiation complex, and 19q13.1, another probable locus for epidermal differentiation-related gene clusters. One EST located at the chromosome 19q13.1 showed increased expression by calcium treatment, suggesting a novel candidate gene relevant to keratinocyte differentiation. These results demonstrate the complexity of the transcriptional profile of keratinocytes, providing important clues on which to base further investigations of the molecular events underlying keratinocyte differentiation.

ISSN 

1011-8934

Link 

http://dx.doi.org/10.3346/jkms.2010.25.4.619

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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