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Title 

Enhanced production of human serum albumin by fed-batch culture of hansenula polymorpha with high-purity oxygen

Authors 

J K YounL ShangM I KimC M JeongH N ChangM S HahmSang Ki RheeHyun Ah Kang

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2010

Citation 

Journal of Microbiology and Biotechnology, vol. 20, no. 11, pp. 1534-1538

Keywords 

Fed-batch cultureHansenulapolymorphaHigh cell densityHigh-purity oxygen fermentationHuman serum albumin

Abstract 

Fed-batch cultures of Hansenula polymorpha were studied to develop an efficient biosystem to produce recombinant human serum albumin (HSA). To comply with this purpose, we used a high-purity oxygen-supplying strategy to increase the viable cell density in a bioreactor and enhance the production of target protein. A mutant strain, H. polymorpha GOT7, was utilized in this study as a host strain in both 5-l and 30-l scale fermentors. To supply high-purity oxygen into a bioreactor, nearly 100% highpurity oxygen from a commercial bomb or higher t han 93% oxygen available in situ from a pressure swing adsorption (PSA) oxygen generator was employed. Under the optimal fermentation of H. polymorpha with highpurity oxygen, the final cell densities and produced HSA concentrations were 24.6 g/l and 5.1 g/l in the 5-l fermentor, and 24.8 g/l and 4.5 g/l in the 30-l fermentor, respectively. These were about 2-10 times higher than those obtained in air-based fed-batch fermentations. The discrepancies between the 5-l and 30-l fermentors with air supply were presumably due to the higher contribution of surface aeration over submerged aeration in the 5-l fermentor. This study, therefore, proved the positive effect of high-purity oxygen in enhancing viable cell density as well as target recombinant protein production in microbial fermentations.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.0909.09046

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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