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Title 

The effects of various antioxidants on the development of parthenogenetic porcine embryos

Authors 

H S YuhD H YuM J ShinH J KimK B BaeD S LeeH C LeeW K ChangS B ParkS G LeeH D ParkJ H HaByung Hwa HyunZ Y Ryoo

Publisher 

Springer Verlag (Germany)

Issue Date 

2010

Citation 

In Vitro Cellular & Developmental Biology-Animal, vol. 46, no. 2, pp. 148-154

Keywords 

AntioxidantApoptosisEmbryoParthenogenesisPorcine

Abstract 

The major objective of this study was to improve the development rate of parthenogenetic porcine embryos. In this study, the anti-oxidative and anti-apoptotic effects of three antioxidants, β-mercaptoethanol (β-ME), α-tocopherol, and extracellular superoxide dismutase (EC-SOD), were examined on the development of parthenogenetic porcine embryos. The development rate of parthenogenetic porcine embryos to the blastocyst stage was 8.1% for control; 19.1%, 14.6%, and 5.0% for 1, 3, and 5 μM β-ME; 17.2% and 17.5% for 50 and 100 μM α-tocopherol and 12.0% and 4.0% for EC-SOD transgenic mouse embryonic fibroblast (Tg-MEF) and EC-SOD non-transgenic mouse embryonic fibroblast (NTg-MEF) conditioned medium at day 3, respectively. Here, β-ME, α-tocopherol, and EC-SOD Tg-MEF conditioned medium increased the development rate of parthenogenetic porcine embryos to the blastocyst stage (P<0.05). The average number of total cells and apoptotic cells at the blastocyst was analyzed at the optimal conditions of the three antioxidants. The three antioxidants increased the average number of total cells at the blastocyst, and they decreased apoptotic cells at the blastocyst as compared to control without supplementation (P<0.05). When the reactive oxygen species levels in two-cell embryos after 1 μM β-ME and 100 μM α-tocopherol treatment were examined, those were lower than control group (P<0.05). In conclusion, it was found that the three antioxidants, β-mercaptoethanol, α-tocopherol, and EC-SOD Tg-MEF, conditioned medium can play a role as a strong stimulator in the development of parthenogenetic porcine embryos.

ISSN 

1071-2690

Link 

http://dx.doi.org/10.1007/s11626-009-9250-1

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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