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Title 

Improving Pb2+ detection using DNAzyme-based fluorescence sensors by pairing fluorescence donors with gold nanoparticles

Authors 

Joong Hyun KimS H HanBong Hyun Chung

Publisher 

Elsevier

Issue Date 

2011

Citation 

Biosensors & Bioelectronics, vol. 26, no. 5, pp. 2125-2129

Keywords 

DNAzymeFluorescence energy transferGold nanoparticlesLeadMultiplexingSensor

Abstract 

For previously reported fluorescence Pb 2+ sensors, DNAzymes have lead to a significant increase in Pb 2+ detecting sensitivity and specificity. However, these sensors suffer from incomplete fluorescence quenching and require additional steps for annealing DNAzymes and substrates as well as for removing the uncoupled substrates. In this study, we successfully overcome these issues by immobilizing the substrate nucleic acids on gold nanoparticles through thiol linkages. The immobilization of the substrate molecules to the gold nanoparticles lead to almost-complete fluorescence quenching and fast Pb 2+ detection, without altering the Pb 2+ specificity of the DNAzymes. After optimizing the concentration of DNAzymes, reaction time and pH, we could detect Pb 2+ as low as 5nM within 20min without the preliminary and the post treatments. Considering the multi-color-fluorescence quenching capability of gold nanoparticles and the to-be-developed functional nucleic acids for other metal ions, this study could extend the application of DNAzymes to the detection of multiple heavy metal ions.

ISSN 

0956-5663

Link 

http://dx.doi.org/10.1016/j.bios.2010.09.018

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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