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Title 

A DNA microarray for identification of selected Korean birds based on mitochondrial cytochrome c oxidase i gene sequences

Authors 

I H ChungHye Sook YooJ Y EahH K YoonJ W JungS Y HwangC B Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2010

Citation 

Molecules and Cells, vol. 30, no. 4, pp. 295-301

Keywords 

birdscytochrome c oxidase IDNA barcodingKoreamicroarraymolecular probes

Abstract 

DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridization, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds. ? 2010 The Korean Society for Molecular and Cellular Biology and Springer Netherlands.

ISSN 

1016-8478

Link 

http://dx.doi.org/10.1007/s10059-010-0118-8

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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