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Title | O-chain structure from the lipopolysaccharide of the human pathogen Halomonas stevensii strain S18214 |
Authors | G Pierettia; S Carilloa; Kang-Kyu Kim; Keun Chul Lee; Jung-Sook Lee; R Lanzettaa; M Parrillia; M M Corsaroa |
Publisher | Elsevier |
Issue Date | 2011 |
Citation | Carbohydrate Research, vol. 346, no. 2, pp. 362-365 |
Keywords | Halomonas; Lipopolysaccharide; NMR spectroscopy; Structure elucidation |
Abstract | Halomonas stevensii is a Gram-negative, pathogenic, moderately halophilic bacterium isolated from the blood of a renal care patient. It optimally grows at 30-35 °C at pH 8-9 and at a sea salt concentration ranging from 3.0% to 7.5%. Gram-negative bacterial infections are closely associated with the presence of the lipopolysaccharides (LPSs) on the outer membrane. These molecules consist of three regions covalently linked: the glycolipid (lipid A), the oligosaccharide region (core region), and the O-specific polysaccharide (O-chain, O-antigen). O-antigen seems to play an important role in the colonization step (adherence) and the ability to bypass host defense mechanisms. For this reason the structure elucidation of the O-chain repeating unit is important to improve knowledge about the role of LPS in the host-pathogen interaction. In this paper, we report the complete structure of the O-chain from the LPS of H. stevensii. The bacterial cells were cultivated and LPS was extracted by the PCP (phenol-chloroform-petroleum ether) method. After mild acid hydrolysis, the lipid A was removed by centrifugation and the obtained polysaccharide was analyzed by means of chemical analysis and one- and two-dimensional NMR spectroscopy giving the following structure |
ISSN | 0008-6215 |
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Registered Date |
2017-04-19 |
