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Title 

Analysis of microbial community during the anaerobic dechlorination of PCE/TCE by DGGE

Authors 

Byung-Hyuk KimDae-Hyun ChoY SungChi-Yong AhnByung Dae YoonS C KohHee-Mock OhHee-Sik Kim

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

2010

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 38, no. 4, pp. 448-454

Keywords 

BioremediationDGGEMicrobial communityPCETCE

Abstract 

This study investigated the effect of PCE and TCE as electron acceptors on the bacterial composition of dechlorinating communities. The enrichment cultures reductively dechlorinating PCE and TCE were developed from three environment samples using acetate as electron donor. The cultures were prepared by sequential enrichment, which was seeded with sediment and dredged soil. Denatured gradient gel electrophresis (DGGE) of 16S rRNA gene fragment was used to compare the microbial communities of these three enrichment cultures. After incubation for 4 weeks, the removal efficiencies of PCE and TCE were highest from Yeocheon site (87.37% and 84.46%, respectively). PCE and TCE as electron acceptors affected the bacterial diversity and community profiles in the enrichment cultures. DGGE analysis showed that the dominant bacteria in PCE and TCE enrichment were belonged to Clostridium sp., Desulfotomaculum sp., and uncultured bacteria.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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